CASE CLOSED … what really happened in the 2001 anthrax attacks?

* Dugway shipped 175 ml Ames spores for irradiation on August 30, 2000 and 340 ml Ames spores for irradiation on Jun 27, 2001

Posted by Lew Weinstein on October 5, 2015

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22 Responses to “* Dugway shipped 175 ml Ames spores for irradiation on August 30, 2000 and 340 ml Ames spores for irradiation on Jun 27, 2001”

  1. DXer said

    Now study the records that I have not uploaded relating to the shipment on August 30, 2000.

    Relatedly, on August 28, 2000, John Ezzell obtained 40 ml of virulent Ames from Flask 1029 for a DARPA mass spec project with JHU/APL See FD 302 sub-302, #3605, dated 1/11/2006

  2. DXer said

    Gamma-irradiated Ames was used at U.S. Army Edgewood under Bio-Safety 1 (BSL-1).

    Did Dr. Lloyd Larsen send the Stern, Ames and Vollum to Dr. John Ezzell in August 2000 and June 2001 for Army-funded biodetection and decontamination experiments at Aberdeen in 2000 and 2001?

    The flow cytometry of Bacillus anthracis spores revisited †
    • Peter J. Stopa*
    http://onlinelibrary.wiley.com/doi/10.1002/1097-0320(20001201)41:4%3C237::AID-CYTO1%3E3.0.CO;2-3/full

    Article first published online: 13 NOV 2000

    Work with gamma-irradiated spores of BA and other strains of Bacillus was performed at the U.S. Army Edgewood Chemical and Biological Center (ECBC) under Bio-Safety Level 1 (BSL-1) practices. Work that required the use of live BA spores was performed under Bio-Safety Level 3 (BSL-3) practices at the Baker Lifesciences Test Facility (BLTF), U.S. Army Dugway Proving Ground.

    Gamma-Irradiated spores of BA (strains: Sterne, Ames, and Vollum) were obtained from Dr. John Ezzell of the U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID). …

    Live spores of BA were provided by Dr. Lloyd Larsen (BLTF) for experiments that were conducted under BSL-3 conditions at Dugway Proving Ground.

    For cross-reactivity studies, Bacillus globigii (BG; now known as Bacillus subtilis var. niger) was obtained from Dr. Bruce Harper (BLTF). BG was supplied as either a dry powder or as spore suspensions in water.

    The U.S. Army Edgewood Chemical and Biological Center, 5183 Blackhawk Road, AMSSB-RRT-DD E3160, Aberdeen Proving Ground, MD 21010-5424 …

  3. DXer said

    These spores were NEVER irradiated. Were they? Weren’t they, in fact, ordered by Bruce Ivins? And didn’t he provide the four vials for seed stock?

    Weren’t the spores then produced by Dugway and shipped to Bruce Ivins and a colleague on June 27, 2001? They weren’t ever radiated, were they?

    Ivins, Bruce E Dr USAMRIID
    Ames spores Sunday, May 06, 2001 4:33:32 PM
    (b) (6)
    (b) (6)
    I believed that you handled the contract with Dugway a few years ago, when we had them produce several lots of Ames spores for us. Do you still have the contract, or do you know who would have it. It would be very helpful to refer to it, because we will soon be needing more Ames spores to replace those which we have been using. Another contract with Dugway would provide us with those spores.
    Thanks for the information. I hope you have the contract or can get a copy of it. – Bruce

    Ivins, Bruce E Dr USAMRIID
    Spore contract with Dugway Monday, May 07, 2001 12:48:23 PM
    (b) (6)
    (b) (6)
    Do you have a copy of a contract between us and Dugway, about 1996 or 1997, in which they made several lots of Bacillus anthracis Ames spores for us? I think we paid them about $50,000, but I’m not sure. Anyway, if you could find it and I could have a copy of it, it would be great. We are in need of more spores and they could make them. If there is something to pattern a new agreement after, it would be very helpful. Thanks!
    – Bruce

    Monday, May 07, 2001 7:16 PM To: Ivins (E-mail) Subject: spores
    Bruce, Got your callback too late in the day to get back with you. On the
    paperwork for the spore production: I will FAX some documents that I have located tomorrow for your use in putting together the appropriate request. My boss says let’s (DPG) go ahead with the production when you’re ready. So it looks good from this end if and when you’re good to go.

    Hi,
    Ivins, Bruce E Dr USAMRIID
    Ames spore – production Tuesday, May 08, 2001 2:51:49 PM
    (b) (6)
    (b)
    (6)Thanks again for your information that you faxed to me. I’m going to start rewriting a new statement of work based on the old one. I’ll forward it to you and your people for their comments, additions, price, etc.
    – Bruce

    Hi,
    Ivins, Bruce E Dr USAMRIID
    Contract Tuesday, May 08, 2001 10:56:06 AM
    (b) (6)
    (b)
    (6)I received your FAX. Thanks! Perhaps you could talk to the appropriate people there and ask if the agreement (Contract? CREDA?) would be acceptable as written, with whatever increase in funds is deemed appropriate. I’ll take this to the contract people here and get started.
    Hope you have had a nice spring. – Bruce

    Hi,
    Ivins, Bruce E Dr USAMRIID
    Ames spore – production Tuesday, May 08, 2001 2:51:49 PM
    (b) (6)
    (b)
    (6)Thanks again for your information that you faxed to me. I’m going to start rewriting a new statement of work based on the old one. I’ll forward it to you and your people for their comments, additions, price, etc.
    – Bruce

    :
    Ivins, Bruce E Dr USAMRIID
    Spores Monday, May 21, 2001 3:57:39 PM
    (b) (6)
    (b) (6)
    (b)
    Hi, Enclosed is a statement of work for Ames spore production based on the one you sent me recently, which was used in 1997. Could you please look it over, then have whoever there needs to go over it do so? Of course, the dollar amount will have gone up, and if there are other changes, please make them. Then if you could send it back to me, I’ll turn it over to who will probably go ahead and OK it as is, since it’s based on the previously approved one.
    Thanks, and hope you’re having a great spring! – Bruce

    [In a subsequent email Dugway corresondent asked if the December 2002 was a mistake — given that Dugway would be able to complete the spores in 2001.]

    Weren’t the spores then produced by Dugway and shipped to Bruce Ivins and a colleague on June 27, 2001? If they were, they weren’t ever radiated, were they?

    • DXer said

      For the sake of completeness, I need to place the same caveat here also:

      I’m now skeptical or at least uncertain that the June 27, 2001 relates to the Dugway-USAMRIID, 21 May 2001 Statement of Work. My present skepticism is due to June 25, 2001 emails where Ivins was hoping to get Dugway paid so they could start work. I just don’t know yet.

      The wonderful USAMRMC officer is going to encourage those who were tasked with looking for documents to look harder.

  4. DXer said

    Truth Official Trailer #1 (2015) Cate Blanchett, Robert Redford Drama Movie HD

    Zero Media

  5. DXer said

    Was the 340 ml. of genetically matching virulent Ames shipped from Dugway to USAMRIID in June 2001 tested for the genetically distrinctive subtilis contaminant? Or did it go missing?

  6. DXer said

  7. DXer said

    Update:

    I apologize for the thread below about the number — my eyesight was faulty. The last four digits are 4768.

    So the commercial number to call would be 301-619-4768.

    That gets you to the Radiation Safety Number. Whether it was the phone or fax back then, I don’t know yet. But you can just call the office and I’m sure the office will be very helpful complying with FOIPA given the outstanding tasked pending at USAMRIID.

    Besides, we owe Ottile Lundgren to find out what happened to the 340 ml. shiipped from Dugway for irradiation in June 2001. Because we know — US Taylor admits — that the anthrax did not come directly from Ivins’ Flask 1029.

    • DXer said

      Currently the point of contact is:

      Josephine S. Esteban-Trexler USAMRIID
      Radiation Safety Officer

      Point of contact is undersigned at 301-619-4626, FAX 301-619-4768 or email Josephine.S.Esteban-Trexler /at/ us.army.mil.

      Who was the Radiation Safety Office in June 2001 at the time of the shipment of 340 ml b. anthracis Ames to USAMRIID?

    • DXer said

      Now let’s turn to the “Review Committee Report: Inadvertent Shipment of Live Bacillus anthraces spores by DoD,” July 13, 2015.

      Dugway also estimated a 2-3% failure rate historically but their own (incomplete) records showed a 21% failure rate:

      The DOD Review states:
      “During the discussion with the technical staff, there were inconsistent answers about how often irradiated samples test positive. One researcher used a generic answer of 2-3% (no documentation was provided)…”

      Irradiation Methodology:
      USAMRIID conducted kill curve studies on Ames and Colorado strains of BA, more than a decade ago. The study subjected samples to gamma radiation doses up to 40 kGy with the results indicating spore inactivation at 20 kGy. For inactivation purposes, the decision was made to use 40 kGy as the dose for spores. Earlier in this report we showed that the current operational procedures used by USAMRIID (and all other DoD laboratories) are outside of the historical experimental kill curves produced at USAMRIID and captured in published research.7,17-20

      “Review Committee Report: Inadvertent Shipment of Live Bacillus anthraces spores by DoD,” July 13, 2015

      To conduct the procedure, the radiation safety officer (RSO) calculates the position of the container of spores to be irradiated in the chamber, as well as the time of exposure to achieve the necessary dose, factoring in the age of the radiation source (Cobalt-60). USAMRIID routinely freezes their samples and maintains this state by employing an ice/dry-ice bath in which the samples sit throughout the irradiation. Independent verification of the dose received is not performed. USAMRIID does not maintain control charts to identify the frequency of off-normal operations by their irradiation instruments.”

      “Review Committee Report: Inadvertent Shipment of Live Bacillus anthraces spores by DoD,” July 13, 2015, p. 27.

      As I said, Dugway also estimated a 2-3% failure rate historically but their own (incomplete) records showed a 21% failure rate. It’s odd that the FBI officials leading the DOD inquiry didn’t pass on the news that the report to the FBI was that Dugway Ames shipped to USAMRIID had been unsuccessfully irradiated.

      “Preparations of Inactivated BA Spores:

      DPG produces inactivated biological agents in support of the DoD Critical Reagents Program. These materials are provided to customers to support research and development, quality assurance, and test and evaluation activities. When the committee asked “how often do irradiated samples fail the viability test?” the answer was about 2-3%. DPG communicated that no root cause analysis was performed on these anomalies. At the end of our discussion, the committee members asked for documentation on failed viability tests of irradiated samples. The documentation provided shows that since 2012, there have been a total of 19 BA inactivations. From these, four samples failed the viability test (21% failure rate). About 10% were attributed to a failing turntable in the irradiator. Lack of supporting documents for many of the answers to critical questions during the committee’s visit pointed to poor record keeping in a critical production laboratory.”

      “Review Committee Report: Inadvertent Shipment of Live Bacillus anthraces spores by DoD,” July 13, 2015, p. 31.

      Will the CDC similarly truncate its analysis and not reach the most relevant shipments of Dugway of all — the shipment of 330 ml. of Ames (reasonably understood to have been genetically identical to that killed five people).

      Doug Beecher: Doug, help Vahid and Christian out here by sorting things through in a principled manner.

      Give effect to the DOD’s mantra of transparency.

      • DXer said

        Irradiation failure occurs when large quantities are done at the same time. For example, tikka chicken from Costco… when I try to do both packages at once in the microwave.

        Or 330 ml. of virulent Ames anthrax. Safety Radiation Officer. Where are the notes of the sterility check? Where are the radiation forms? Please give them to the USMRMC FOIPA Officer when she comes calling. CDC has given its blessing.

  8. DXer said

    The contact for the 340 ml of virulent Ames shipped from Dugway to USAMRIID in June 2001 was DSN 343-4788.

    Don’t be shy, USAMRMC. Pick up the phone and call the contact to locate the relevant documents relating to the shipment.

    • DXer said

      That 343 is the Ft. Detrick prefix. For example, the main DNS number for Ft. Detrick is DNS 343-1110.

      Here is a current phone directory.

      http://www.detrick.army.mil/phbook/phbook.pdf

      I might have to go to a different computer to get the phone directory from 2002.

      Who was at 343-4788?

      Will my favorite FOIPA officer in the entire world let her fingers do the walking? Will the person tasked to find the documents pertaining to the shipment?

      http://www.detrick.army.mil/phbook/phbook.pdf

      • DXer said

        Here is a 2004 Ft. Detrick telephone book.

        https://web.archive.org/web/20040325070230/http://www.detrick.army.mil/phbook/phbook.pdf

        But turning to look at it, I see that DNS numbers are not in it.

        So I think it will take someone in the Army or more learned than me to call and ask about the documents relating to the 340 ml of virulent Ames shipped to USAMRIID in June 2001.

      • DXer said

        The Phone number for the Bacteriology Division/ Point of Contact Bruce Ivins, was listed as DSN 343-7341. So I don’t think he was the contact for the mysterious shipment of 340 ml in June 2001.

        A Facsimile sheet that Dr. Ivins used gave FAX Phone Numbers: DSN 343-2152 and Commercial (301) 619-2152.

        And elsewhere I see that USAMRMC Public Affairs Office has the numbers 301-619-7549 or DSN 343-7549.

        So (forgive my lack of experience with the military), I believe that what you do is take an ordinary phone number is the same last four digit of the DNS number.

        So that means the contact’s “Commercial Number”, then, it seems, would be 301 619-4788.

        Whose number was that in 2001?

    • DXer said

      Or maybe the contact for the 340 ml of virulent Ames shipped from Dugway to USAMRIID in June 2001 was DSN 343-4768 — that is, maybe the last two digits are “68” rather than “88”. In the full pdf available online, another listing provides that contact number and with that entry I’m leaning towards it being a “68.” I found the “88” number as a residence listing a block or two away from Detrick but not in the 2004 phone book. So now I’ll check for the “68” number.

      ______

      “He can’t do that to our pledges. Only we can do that to our pledges.” — Animal House

  9. DXer said

    Is this 340 ml. shipped from Dugway in June 2001 to USAMRIID the anthrax shipped to USAMRIID that was rumored to have gone missing? Ivins calculated that it would take about 300 ml to make the anthrax used in the mailed letters. (at the concentration of Flask 1029).

    Was the 340 ml. shipped from Dugway to USAMRIID in June 2001 for irradiation ever, in fact, shipped back to Dugway? The new happy recipient of the FOIPA tasker is doing some head-scratching.

    The USAMRIID has delayed for many months responding to records relating to Dugway anthrax shipments. Ottilie Lundgren is still dead.

    I had not remembered that Flask 1030 was the seed stock for Flask 1029. It is counterintuitive given that the number 1030 is bigger than 1029. (1030 itself was a mixture and had a silicon signature; it had 3 morphs).

    Four vials from 1030 was sent to Dugway in 1997. Both the FBI and USAMRIID have failed to give me Notebook 3655 (pertaining to Flask 1030) and they are not complying with FOIPA. If they are not produced, the person who continues to keep them from production should be fired as it constitutes an obstruction of justice.

    https://caseclosedbylewweinstein.wordpress.com/2015/10/04/for-the-notebook-describing-usamriids-shipment-of-4-vials-of-ba-ames-to-dugway-which-contained-numerous-entries-by-ivins-see-this-notebook-that-usamriid-refuses-to-produce-despite-repeated-reque/

    So as the snippet from the document above shows, Dugway sent back 340 ml in June 2001 for irradiation.

    Importantly, we know now that USAMRIID radiation was sometimes was ineffective. See 2004 report in pumpkin graphic. After irradiation, it was kept in the cold room near the loading dock. No, I’m not going to tell you what Building. Let former US Attorney Taylor and Vahid Majidi bullshit an answer to a Congressional committee.

    https://caseclosedbylewweinstein.wordpress.com/2015/10/04/in-2004-the-fbi-was-told-that-ivins-handled-irradiation-of-dugway-spores-which-then-were-kept-in-the-cold-room-even-though-irradiation-was-not-always-successful/

    Oh — and there was a Dugway shipment vaguely rumored to have gone missing.

    https://caseclosedbylewweinstein.wordpress.com/2015/07/29/dugway-anthrax-will-it-take-congressional-subpoena-power-to-fill-in-the-blanks-in-the-email-asking-about-weaponized-anthrax-that-came-to-detrick-and-then-was-shipped-out-and-some-was-missing/

    Ivins calculated that it would take 300 ml. to make the anthrax used in the mailings.

    Are there in fact shipment records proving that USAMRIID shipped back the 340 ml of Ames that was sent from Dugway to USAMRIID after irradiation? The wonderful FOIPA official is only hopeful that fresh eyes can be brought to bear under the new tasker.

    I’ve suggested that JAG has iNotebooks 4010 and Notebook 3655 bearing on these issues — lawyers representing the Army would have wanted to see the record so directly related to the so-called “murder weapon”.

    https://caseclosedbylewweinstein.wordpress.com/2015/10/04/dod-inspector-general-staff-judge-advocate-handling-the-subpoena-for-the-lab-notebooks-is-named-in-the-april-2007-correspondence-with-bruce-ivins/

    Is the 340 ml of Ames the source of the so-called “Iraq sample” of powderized anthrax that Ivins was asked to look at? He intentionally mislabeled it and then what was left of the powder went missing when Fellows left.

  10. DXer said

    The shipping of the Dugway Ames to USAMRIID in August 2000 and June 2001 are located here in a list of shipments. Sorry I don’t have a more specific link but you can get to it by scrolling down.

    https://vault.fbi.gov/Amerithrax/Amerithrax%20Part%2036%20of%2059

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