CASE CLOSED … what really happened in the 2001 anthrax attacks?

* Subtilis Contaminant in Anthrax Mailed Fall 2001: Unclassified/For Official Use Only

Posted by DXer on May 5, 2011



7 Responses to “* Subtilis Contaminant in Anthrax Mailed Fall 2001: Unclassified/For Official Use Only”

  1. DXer said

    The WMD head Vahid Majidi now admits that the FBI did not swab the suspect labs for subtilis. He says that the 100 million dollars only goes so far. We are only hearing this 5 years after the fact after he and his colleagues falsely assured the public that the swabbing for subtilis had been done and no match was found.

  2. DXer said


    Impurity Profiling to Match a Nerve Agent to Its Precursor Source for Chemical Forensics Applications. Detail Only Available By: Fraga, Carlos G.; Acosta, Gabriel A. Pérez; Crenshaw, Michael D.; Wallace, Krys; Mong, Gary M.; Colbun, Heather A.. Analytical Chemistry, 12/15/2011, Vol. 83 Issue 24, p9564-9572, 9p; DOI: 10.1021/ac202340uSubjects: Forensic sciences; Cholinesterase inhibitors; Chemicals; Trace analysis; Chemical (except agricultural) and allied product merchant wholesalers; Nerve gases

  3. DXer said

    This work centering around subtilis began in September 2002. The authors are available online to describe the work done in 2001 involving subtilis.

    Edgewood Chemical Biological Center, “Production of Bacillus Spores as a Simulant for Biological Warfare Agents,” Final rept. Sep 2002-Sep 2003

    • DXer said

      How did the oxygren and hydrogen stable isotope ratios in the subtilis contaminant compare to those in the bacillus Ames?

      Kreuzer-Martin, H. W. et al., “Microbe forensi Proceedings of the National Academy of Sciences,” February 4, 2003

      Microbe forensics: Oxygen and hydrogen stable isotope ratios in Bacillus subtilis cells and spores

      • Helen W. Kreuzer-Martin*†,
      • Michael J. Lott*,
      • Janet Dorigan‡, and
      • James R. Ehleringer*
      + Author Affiliations

      • *Stable Isotope Ratio Facility for Environmental Research, Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112; and ‡Central Intelligence Agency, Washington, DC 20505
      • Communicated by Thure E. Cerling, University of Utah, Salt Lake City, UT (received for review September 30, 2002)


      Bacillus subtilis, a Gram-positive, endospore-forming soil bacterium, was grown in media made with water of varying oxygen (δ18O) and hydrogen (δD) stable isotope ratios. Logarithmically growing cells and spores were each harvested from the cultures and their δ18O and δD values determined. Oxygen and hydrogen stable isotope ratios of organic matter were linearly related with those of the media water. We used the relationships determined in these experiments to calculate the effective whole-cell fractionation factors between water and organic matter for B. subtilis. We then predicted the δ18O and δD values of spores produced in nutritionally identical media and local water sources for five different locations around the United States. Each of the measured δ18O and δD values of the spores matched the predicted values within a 95% confidence interval, indicating that stable isotope ratio analyses may be a powerful tool for tracing the geographic point-of-origin for microbial products.

  4. DXer said

    From: Ivins, Bruce E Dr USAMRIID
    Subject: Requested Information
    Date: Friday, March 19, 2004 2:43:07 PM

    I am sending you information requested over the telephone this morning.
    Additional information:

    USAMRIID Notebooks 3655 and 4010 contain information pertaining to Ames spore preparations RMR
    (Reference Material Receipt) 1030 and RMR 1029.

    Our laboratory’s procedure for producing, harvesting and purifying anthrax spores was published:
    Ivins, B. E., . Immunization against anthrax with aromatic compound-dependent (Aro-) Mutants of Bacillus anthracis and with recombinant strains of Bacillus subtilis that produce anthrax protective antigen. Infection and Immunity. 58:303-308.
    Bruce Ivins

  5. DXer said

    Who was Bruce corresponding with about this?

    From: Ivins, Bruce E Dr USAMRIID
    Subject: RE: Yet Another Delta Sterne ?
    Date: Thursday, February 13, 2003 9:33:33 AM
    transformed pPA101 and pPA102 into B. subtilis WB600 (a protease-deficient
    > —–Original Message—–
    >Sent: Thursday, February 13, 2003 9:07 AM
    >To: Ivins, Bruce E Dr USAMRIID
    >Subject: FW: Yet Another Delta Sterne ?
    >Do you have time today to look at the paper and see what it actually says (pPA101 vs. pPA102,
    subtilis vs. anthracis; see my notes in black below). I have to meet with my little three letter friends
    this morning and is in a hurry.

  6. DXer said

    Subtilis, for example, is used in testing the effectiveness of decontamination.

    Sent: Monday, June 24, 2002 6:49 PM
    To: Bruce.Ivins@DET.AMEDD.ARMY.MIL
    Subject: RE: Boxes to be decontaminated
    _____and I have discussed this and we have the following recommendation to avoid
    re-opening your boxes:

    1. Before the boxes are processed we need to perform a trial run to assure
    ourselves that all the contents of the boxes will receive the minimum dose to kill all the spores on a 106
    B. subtilis spore strip.

    2. We need two simulant boxes loaded with similar books/notebooks and
    packaged the same way. The bags and boxes do not need to be tapped shut as we will need access to
    the contents before the run.

    3. We will place 106 B. subtilis spore strips into the boxes at several strategic
    locations, perform an exposure to kill 8-logs of dry spores and withdraw the spore strips for a sterility

    4. If this test is successful, we can proceed with the other boxes with spore
    strips on the outside.

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